Glucose Inhibition of Respiration in Ascites Tumor Cells.

The inhibition of respiration by glucose, fructose, and mannose in the Ehrlich mouse ascites tumor.

Inhibition of anaerobic glycolysis in Ehrlich ascites tumor cells by 2-deoxy-D-glucose.

Little is known of the mechanisms by which 2-deoxy-D-glucose (2-DG) inhibits anaerobic glycolysis in yeast cells (1, 9, 13, 14) and in brain, diaphragm, and liver slices (15). This compound also inhibits anaerobic glycolysis in various tumors (15), prolongs the survival time of mice bearing the Krebs ascites carcinoma (2), and causes a decreased rate of growth of several tumors in rats or mice ...

Feedback Inhibition of Purine Biosynthesis in Ascites Tumor Cells*

Feedback inhibition of purine biosynthesis de novo in bacteria has been studied in some detail by Gots and his colleagues (l-4). Other observations have suggested feedback inhibition, but these are complicated by dilution of intermediary pools by the added inhibitor (5-10). This reaction has been studied less extensively in mammalian cells. Sartorelli and LePage (11) and LePage and Jones (12) h...

Metabolism of Ascites Tumor Cells. Ii. Inhibition of Respiration by Glycolyzable and Nonglycolyzable Sugars Phosphorylated by Hexokinase.

The effects of twelve monosaccharides on the oxygen uptake of Krebs-2 ascites carcinoma cells were determined during prolonged incubation. Seven sugars were shown to inhibit respiration. The incubation periods required to induce inhibition were inversely related to the maximum rate of phosphorylation of each sugar by tumor hexokinase. Of these inhibiting sugars, the nonglycolyzable were 2-deoxy...

Effect of various synthetic estrogens on the respiration of ascites tumor cells in vitro.

The effects of the synthetic estrogens diethylstilbesterol, hexosterol, dienesterol, and phloretin were compared with their capacity to inhibit the endogenous respiration of intact Ehrlich ascites, Adenocarcinoma 755 ascites, and Ehrlich-Lettr6 ascites cells. The Ehrlich ascites was found to be far more susceptible to respiratory inhibition than either the Adenocarcinoma 755 or the Ehrlich-Lett...